Researchers develop new method for peptide sequencing based on nanopore sensing technology

This article has been reviewed according to Science X’s editorial process
and policies.
Editors have highlighted the following attributes while ensuring the content’s credibility:


peer-reviewed publication

trusted source


Identification of the 20 amino acids using the FGGCD8 probe. Credit: Nature Methods (2023). DOI: 10.1038/s41592-023-02095-4

× close

Identification of the 20 amino acids using the FGGCD8 probe. Credit: Nature Methods (2023). DOI: 10.1038/s41592-023-02095-4

New protein sequencing technology with improved sensitivity and throughput will bring revolution to proteomics and clinical diagnostics.

In a study published in Nature Methods, a research team led by Prof. Wu Haichen from the Institute of Chemistry of the Chinese Academy of Sciences (CAS), and Prof. Liu Lei from the Institute of High Energy Physics of CAS, together with their collaborators, have developed a new method for peptide sequencing based on host-guest interaction-assisted nanopore sensing.

The history of protein sequencing could be dated back to the determination of the complete amino acid sequence of insulin by Sanger in the 1950s. However, so far, there are only two major methods for protein sequencing, i.e., mass spectrometry and Edman degradation.

During the past few decades, nanopore sensing emerged as the latest “disruptive” single-molecule technique and has gained great success in the new generation of DNA sequencing development. This has inspired scientists to transplant the technology to single-molecule protein sequencing. However, nanopore sequencing of proteins faces tremendous challenges, such as the realization of a unidirectional transport of heterogeneously charged peptide chains through a nanopore, and electrical identification of 20 individual amino acids or their combinations.

In this study, the researchers proposed an alternative sequencing strategy based on an improved host-guest interaction-assisted nanopore sensing technique.

The model peptides were first digested with carboxypeptidases to afford a mixture of amino acids. The next key step was to couple the released amino acids to a FGGCD8⊂CB[7] peptide probe via a covalent linker and then subject the complex to translocation experiments through wildtype α-hemolysin or its mutants.

Finally, the current blockage of each FGGC(X)D8⊂CB[7] peptide was used to identify the amino acid X and their relative abundance was used to determine the order of the enzymatic cleavage, i.e., the sequence of the peptide.

This study serves as a proof-of-concept demonstration for a novel method capable of precisely determining the amino acid sequence of a peptide. While notable limitations persist, this marks a significant advancement and unveils a promising avenue for the future of protein sequencing.

More information:
Yun Zhang et al, Peptide sequencing based on host–guest interaction-assisted nanopore sensing, Nature Methods (2023). DOI: 10.1038/s41592-023-02095-4

Journal information:
Nature Methods

Source link

31 thoughts on “Researchers develop new method for peptide sequencing based on nanopore sensing technology

  1. Do you mind if I quote a couple of your posts as long
    as I provide credit and sources back to your weblog?
    My blog site is in the very same area of interest as yours and my users would really benefit from a lot of the information you
    present here. Please let me know if this ok with you.
    Check these web pages:

  2. It’s a shame you don’t haᴠe a donate button! I’d сertainly ddonate tο this fantastic blog!
    I suppose fⲟr noѡ i’ll settle for bookmarking and
    adding yoսr RSS feed to mу Google account. І liok forward tߋ new updates and ԝill share tһis blog wigh my Facebook ցroup.
    Talk soon!

  3. Just desire to say your article is as astonishing.
    The clarity in your post is just excellent and i could assume you’re an expert on this subject.

    Well with your permission let me to grab your feed to keep updated with forthcoming post.
    Thanks a million and please carry on the enjoyable work.

  4. Mу spouse and I stumbled over һere from a different page and thought I might check things out.
    I like what I see so now i am following you.
    Look forward to loiking іnto your web page again.

Leave a Reply

Your email address will not be published. Required fields are marked *